Detecting cancer biomarkers in blood: challenges for new molecular diagnostic and point-of-care tests using cell-free nucleic acids

In this review, the authors discuss emerging cell-free nanoparticles isolation technologies that demonstrate the potential for being translated into for point-of-care application. The simplicity of sample preparation used in Biological Dynamics AC dielectrophoretic separation enables future applications of molecular diagnostics at point-of-care.

Lewis JM, Heineck DP, Heller MJ. Expert Rev Mol Diagn. 2015 Jul 18. doi: 10.1586/14737159.2015.1069709. 


As we move into the era of individualized cancer treatment, the need for more sophisticated cancer diagnostics has emerged. Cell-free (cf) nucleic acids (cf-DNA, cf-RNA) and other cellular nanoparticulates are now considered important and selective biomarkers. There is great hope that blood-borne cf-nucleic acids can be used for ‘liquid biopsies’, replacing more invasive tissue biopsies to analyze cancer mutations and monitor therapy. Conventional techniques for cf-nucleic acid biomarker isolation from blood are generally time-consuming, complicated and expensive. They require relatively large blood samples, which must be processed to serum or plasma before isolation of biomarkers can proceed. Such cumbersome  sample preparation also limits the widespread use of powerful, downstream genomic analyses, including PCR and DNA sequencing. These limitations also preclude rapid, point-of-care diagnostic applications. Thus, new technologies that allow rapid isolation of biomarkers directly from blood will permit seamless sample-to-answer solutions that enable next-generation point-of-care molecular diagnostics.


Rapid Electrokinetic Isolation of Cancer-Related Circulating Cell-Free DNA Directly from Blood

The authors used Biological Dynamics AC electrokinetic platform to isolate, to elute and to sequence cell-free DNA from 25 μL unprocessed blood from 15 chronic lymphocytic leukemia (CLL) patients and 3 healthy individuals. The complete process, blood to PCR, required <10 min.

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