The authors used Biological Dynamics AC electrokinetic platform to isolate, to elute and to sequence cell-free DNA from 25 μL unprocessed blood from 15 chronic lymphocytic leukemia (CLL) patients and 3 healthy individuals. The complete process, blood to PCR, required <10 min.
Rapid Electrokinetic Isolation of Cancer-Related Circulating Cell-Free DNA Directly from Blood
Avery Sonnenberg, Jennifer Y. Marciniak, Laura Rassenti, Emanuela M. Ghia, Elaine A. Skowronski, Sareh Manouchehri, James McCanna, George F. Widhopf II, Thomas J. Kipps and Michael J. Heller
Background: Circulating cell-free (ccf) DNA is becoming an important biomarker for cancer diagnostics and therapy monitoring. The isolation of ccf-DNA from plasma as a “liquid biopsy” may begin to replace more invasive tissue biopsies for the detection and analysis of cancer-related mutations. Conventional methods for the isolation of ccf-DNA from plasma are costly, time-consuming, and complex, preventing the use of ccf-DNA biomarkers for point-of-care diagnostics and limiting other biomedical research applications.
Conclusion: Rapid isolation of cell-free DNA directly from a drop of blood will advance disease-related biomarker research, accelerate the transition from tissue to liquid biopsies, and enable point-of-care diagnostic systems for patient monitoring.