In this article, the authors used Biological Dynamic ACE technology to demonstrate the ability to isolate glioblastoma exosomes from undiluted plasma and detect proteins and RNA bound to the exosomes.Read More
In this article, the authors demonstrate feasibility of detecting cancer mutations in eluted cell-free DNA that was isolated from the plasma of chronic lymphocytic leukemia (CLL) patients using Biological Dynamics platform.
Sareh Manouchehri, Stuart Ibsen, Jennifer Wright, Laura Rassenti, Emanuela M Ghia, George F Widhopf, Thomas J Kipp & Michael J Heller
Aim: Circulating cell free (ccf) DNA contains information about mutations affecting chronic lymphocytic leukemia (CLL). The complexity of isolating DNA from plasma inhibits the development of point-of-care diagnostics. Here, we introduce an electrokinetic method that enables rapid recovery of DNA from plasma. Materials & methods: ccf-DNA was isolated from 25 µl of CLL plasma using dielectrophoresis. The DNA was used for PCR amplification, sequencing and analysis. Results: The ccf-DNA collected from plasma of 5 CLL patients revealed identical mutations to those previously identified by extracting DNA from CLL cells from the same patients. Conclusion: Rapid dielectrophoresis isolation of ccf-DNA directly from plasma provides sufficient amounts of DNA to use for identification of point mutations in genes associated with CLL progression.
In this article, the authors used Biological Dynamic technology demostrated ability to isolate wide range of drug delivery nanoparticles, including low-density nanoliposomes directly from blood.Read More
The authors used Biological Dynamics AC electrokinetic platform to isolate, to elute and to sequence cell-free DNA from 25 μL unprocessed blood from 15 chronic lymphocytic leukemia (CLL) patients and 3 healthy individuals. The complete process, blood to PCR, required <10 min.Read More
This study demonstrated the potential clinical relevance of this Biological Dynamics technology for diagnostic applications as a a “seamless sample-to-answer” technique for the rapid detection of cfc-DNA and nanoparticulate biomarkers directly from blood and other complex biological samples.The authors showed rapid isolation and detection of SYBR Green stained cell-free DNA from 20 µL whole blood samples from Chronic Lymphocytic Leukemia (CLL) patients.
Overall the results of this study support the enormous potential of DEP as a “seamless sample-to-answer” technique for the rapid detection of cfc-DNA and nanoparticulate biomarkers directly from blood and other complex biological samples.Read More