Dielectrophoretic recovery of DNA from plasma for the identification of chronic lymphocytic leukemia point mutations

In this article,  the authors demonstrate feasibility of detecting cancer mutations in eluted cell-free DNA that was isolated from the plasma of chronic lymphocytic leukemia (CLL) patients using Biological Dynamics platform.

Sareh Manouchehri, Stuart Ibsen, Jennifer Wright, Laura Rassenti, Emanuela M Ghia, George F Widhopf, Thomas J Kipp & Michael J Heller


ABSTRACT

Aim: Circulating cell free (ccf) DNA contains information about mutations affecting chronic lymphocytic leukemia (CLL). The complexity of isolating DNA from plasma inhibits the development of point-of-care diagnostics. Here, we introduce an electrokinetic method that enables rapid recovery of DNA from plasma. Materials & methods: ccf-DNA was isolated from 25 µl of CLL plasma using dielectrophoresis. The DNA was used for PCR amplification, sequencing and analysis. Results: The ccf-DNA collected from plasma of 5 CLL patients revealed identical mutations to those previously identified by extracting DNA from CLL cells from the same patients. Conclusion: Rapid dielectrophoresis isolation of ccf-DNA directly from plasma provides sufficient amounts of DNA to use for identification of point mutations in genes associated with CLL progression.

Link to abstract

Rapid Electrokinetic Isolation of Cancer-Related Circulating Cell-Free DNA Directly from Blood

The authors used Biological Dynamics AC electrokinetic platform to isolate, to elute and to sequence cell-free DNA from 25 μL unprocessed blood from 15 chronic lymphocytic leukemia (CLL) patients and 3 healthy individuals. The complete process, blood to PCR, required <10 min.

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Dielectrophoretic Isolation and Detection of cfc-DNA Nanoparticulate Biomarkers and Virus from Blood

This study demonstrated the potential clinical relevance of this Biological Dynamics technology for diagnostic applications as a a “seamless sample-to-answer” technique for the rapid detection of cfc-DNA and nanoparticulate biomarkers directly from blood and other complex biological samples.The authors showed rapid isolation and detection of SYBR Green stained cell-free DNA from 20 µL whole blood samples from Chronic Lymphocytic Leukemia (CLL) patients.

Overall the results of this study support the enormous potential of DEP as a “seamless sample-to-answer” technique for the rapid detection of cfc-DNA and nanoparticulate biomarkers directly from blood and other complex biological samples.

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